This application note describes a fully automated analysis method for selected equine doping compounds in equine urine. A GERSTEL Mul tiPurpose Sampler (MPS) with Disposable Pipette Extraction (DPX) option is employed for extraction and cleanup. After gas chromatographic separation the analytes are detected by a triple-quadrupole mass spectrometer (QqQ-MS). The method is rugged, provides an adequate cleanup of the complex sample matrix and shows good limits of detection, from below 0.1 to just under 10 ng/mL for the various analytes that are determined.

The use of performance enhancing substances predates the beginning of ancient Olympics in Greece. However, until the 20th century the word “doping” had not been widespread.